Figure 1.
Figure 1.

Workflow for the application of Roche 454 pyrosequencing in combination with genomic reduction and the computational pipeline DIAL (de novo identification of alleles) (Ratan et al., 2010) to identify contigs and single nucleotide polymorphisms (SNPs) from 16 barley samples. F, forward; R, reverse; RLMID, Rapid Library Multiplex Identifier; SFF, standard flowgram format.

 


Figure 2.
Figure 2.

Single nucleotide polymorphism (SNP)- and simple sequence repeat (SSR)-based genetic relationships of the 16 barley samples inferred with the principle component analysis (PCA) (A and D), the NeighborNet (Bryant and Moulton, 2004) of SplitsTree4 (Huson and Bryant, 2006) (B and E) and the maximum clade credibility (MCC) tree of BEAST (Drummond and Rambaut, 2007) with tree prior as exponential growth (C and F). The sample labels are given in Table 1.