View Full Table | Close Full ViewTable 1.

Populations segregating for soybean cyst nematode resistance tested with markers to identify genetic recombination events close to rhg1-b.

 
Cross no. Female parent Source of female parent Male parent Source of male parent
1 LD02–5320 (R) Univ. of Illinois 99805 (S) Dairyland Seed
2 LD02–5025 (R) Univ. of Illinois LG03–1672 (S) USDA-ARS
3 LD02–5025 (R) Univ. of Illinois LG00–3372 (S) USDA-ARS
4 IA3023 (S) Iowa State University LD01–7323 (R) Univ. of Illinois
R, resistant parent in the cross.
S, susceptible parent in the cross.



View Full Table | Close Full ViewTable 2.

Positions of simple sequence repeat (SSR), insert-deletion (INDEL), and single nucleotide polymorphism (SNP) markers, marker data, and recombination points for plants or lines selected for having recombination near the rhg1-b gene on chromosome 18. The base pair (bp) positions are based on the Williams 82 whole genome sequence (Glyma 1.01 build) and the centimorgan (cM) positions are based on the published soybean genetic map (Hyten et al., 2010). The telomeric end is on top and the centromic end of the chromosome is on the bottom of the map. Note that “S”, “H”, and “R” refer to the marker genotype of the plant that the lines were derived from and not the SCN phenotype of the line noted at the top of the column. An arrow is placed in the genetic interval carrying the inferred recombination breakpoint; arrow points toward side of recombination breakpoint associated with the SCN resistance phenotype (inferred from data in Table 4).

 



View Full Table | Close Full ViewTable 3.

Response of differential soybean genotypes to the PA3 soybean cyst nematode isolate used in each experiment.

 
Number of cysts of the susceptible cultivar Macon Female index
Lines tested 1 Peking 2 PI 88788 3 PI 90763 4 PI 437654 5 PI 209332 6 PI 89772 7 PI 548316
2–027, 2–124, 3–161 32 2 5 1 0 22 0 0
1–080, 1–144, 4–106 112 1 4 0 0 10 1 11
3–76, 4–67, 2–116, 2–130, 2–14, 2–137 153 0 20 0 0 30 0 37
3–76–1, 4–67–1 21 5 ND 1 ND ND 0 ND
3–76–2, 4–67–2 612 2 19 0 0 32 1 33
3–76–3, 4–67–3 146 3 32 0 0 51 1 39
4–63, 1–50, 1–218, 1–184, 2–63 330 1 26 0 0 39 0 31
ND, Not determined because the differential plants did not germinate in the test.



View Full Table | Close Full ViewTable 4.

Marker and soybean cyst nematode resistance results for F2:3, F3:4, or F4:5 lines used to position rhg1-b.

 
Female index
Line Recombination point No. of plants tested Marker used in F test R H S P > F R2
2–124 1 33 Satt309 22 53 73 0.0010 0.37
3–161 2 30 ss107914244 93 73 25 0.14 0.13
1–80 3 38 Satt309 5 31 81 < 0.0001 0.82
4–106 3 38 Satt309 8 29 62 < 0.0001 0.72
2–27 4 27 ss107914244 60 58 47 0.52 0.05
1–144 5 37 ss107914244 61 49 50 0.15 0.10
3–76 6 22 Satt309 91 67 94 0.22 0.14
3–76–1 6 36 Satt309 36 49 43 0.24 0.08
3–76–2 6 28 Satt309 72 80 83 0.40 0.07
3–76–3 6 39 Satt309 93 91 109 0.13 0.11
4–67 7 34 Sat_168 36 65 95 < 0.0001 0.58
4–67–1 7 35 Sat_168 26 52 74 < 0.0001 0.49
4–67–2 7 20 Sat_168 34 60 77 < 0.0001 0.73
4–67–3 7 36 Sat_168 38 62 91 < 0.0001 0.70
4–63 8 37 Sat_168 44 39 44 0.68 0.02
2–130 9 40 Satt309 38 57 70 0.02 0.19
2–14 9 40 Satt038 62 66 74 0.52 0.03
2–137 9 40 Satt309 29 44 69 < 0.0001 0.38
1–50 10 40 Sat_168 34 66 103 < 0.0001 0.46
1–218 11 17 BARCSOYSSR_18_0083 76 81 89 0.77 0.04
1–184 12 31 BARCSOYSSR_18_0083 71 79 84 0.88 0.02
2–63 13 37 Sat_168 48 84 102 < 0.0001 0.55
Mean female index for the plants in each line that were predicted on the basis of the genetic markers Satt309, Sat_168, Satt038, or ss107914244, as noted in previous column, to be homozygous for allele from resistant parent (R), heterozygous (H), or homozygous for allele from susceptible parent (S).
Position of the recombination on Table 2.
§Number of plants in the line tested in the greenhouse for SCN resistance.
Significance level of the marker association.
#R2 value of the marker association.