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This article in SSSAJ

  1. Vol. 61 No. 6, p. 1679-1685
     
    Received: Mar 18, 1996
    Published: Nov, 1997


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doi:10.2136/sssaj1997.03615995006100060020x

Differentiation of Clover Rhizobium Isolated from Biosolids-Amended Soils with Varying pH

  1. A. M. Ibekwe,
  2. J. S. Angle ,
  3. R. L. Chaney and
  4. P. van Berkum
  1. Dep. of Agronomy, Univ. of Maryland, College Park, MD 20742
    USDA-ARS Environmental Chemistry Lab., Beltsville, MD 20705
    USDA-ARS Soybean and Alfalfa Research Lab., Beltsville, MD

Abstract

Abstract

Metal contamination may alter the diversity of microbes residing in soil. The genetic structure and phenotypic characteristics of clover Rhizobium isolated from contaminated and control soils were compared. Plant infection and symbiotic competence tests were used for phenotypic characterization. Variation across isolates in fingerprint patterns determined with primers for repetitive extragenic palindromic (REP) sequences and the polymerase chain reaction (PCR) were used for genetic characterization. Two phenotypic groups of effective and ineffective isolates were identified using the symbiotic effectiveness test. Soil pH was the primary factor influencing this phenotypic characteristic. Effective isolates were associated with higher soil pH and ineffective isolates were associated with lower soil pH regardless of soil metal content. The isolates were genetically diverse. The variation of isolates from the different soils overlapped, indicating that neither the heavy metals nor the low soil pH resulted in the selection of a single genotype. Isolates from the most heavily contaminated soils were more variable than isolates from control soils. Soil pH, and not heavy metal content, was important in the selection of rhizobia that formed ineffective N2-fixing symbioses.

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