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This article in SSSAJ

  1. Vol. 56 No. 3, p. 762-765
     
    Received: Apr 24, 1991
    Published: May, 1992


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doi:10.2136/sssaj1992.03615995005600030014x

Partitioning Dissolved Inorganic and Organic Phosphorus Using Acidified Molybdate and Isobutanol

  1. K. Jayachandran,
  2. B. A. D. Hetrick and
  3. A. P. Schwab 
  1. Dep. of Plant Pathology
    Dep. of Agronomy, Throckmorton Hall, Kansas State Univ., Manhattan, KS 66506-5502

Abstract

Abstract

Quantification of P-mineralization rate in soil using isotopic dilution requires that soluble inorganic 32P (32Pi) be determined without interference from soluble organic 32P (32Po). Therefore, a method is needed to partition 32Pi and 32Po into physically separate solutions before radiation counting. A previously suggested method of extracting Pi into isobutanol with acidified molybdate was tested for efficiency of separation from Po. Inorganic P as KH2PO4 was completely recovered in the isobutanol phase with acid molybdate. Organic P (glycerophosphate) remained in the aqueous phase during the extraction. No Po hydrolysis was observed when sodium phytate, glycerophosphate, ribonucleic acid, adenosine 2′- and 3′-monophosphate, and cytidine 2′- and 3′-monophosphate were extracted, but detectable amounts of adenosine 5′-triphosphate hydrolyzed. This technique was tested on four Kansas soils, and the levels of inorganic and organic P obtained by this method were the same as those detected by conventional analyses.

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