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This article in SSSAJ

  1. Vol. 53 No. 6, p. 1707-1711
     
    Received: Oct 7, 1988
    Published: Nov, 1989


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doi:10.2136/sssaj1989.03615995005300060016x

Diffusion Method To Prepare Soil Extracts For Automated Nitrogen-15 Analysis

  1. P. D. Brooks ,
  2. J. M. Stark,
  3. B. B. McInteer and
  4. T. Preston
  1. Dep. of Soil Science, Univ. of California, Berkeley, CA 94720
    Isotope Services Los Alamos, NM 87544
    Scottish Univ. Research and Reactor Centre, East Kilbride G75 OQU, Scotland

Abstract

Abstract

A diffusion method that requires less labor than distillation methods was developed for preparing soil KCl extracts for 15NH+4 and 15NO-3 analyses. The procedure is ideal for preparing samples having low N mass (50–200 µg N) with no cross contamination. The method uses 140 mL disposable specimen containers to hold the sample, and 7-mm diam, disks of acidified GF/D glass fiber filter paper on stainless steel wire as the acid trap. Devarda's alloy and MgO are used as the reductant and base respectively. Complete diffusion for 40 to 60 mL of sample takes 6 d at room temperature with no shaking. Paired t-tests comparing analyses of soil extracts of varying 15N enrichment by this method and steam distillation showed no significant difference (p = 0.64). Use of KHSO4 as the trapping acid allows the paper disks to be placed in a Sn capsule and analyzed by direct combustion mass spectrometry. Alternately, H2SO4 can be used and the disks analyzed by an automated NaOBr method. The disks can be easily dried in a desiccator over H2SO4 and mailed for 15N analysis by service laboratories.

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