Comparison of Methods to Extract Spores of Vesicular-Arbuscular Mycorrhizal Fungi
- Garriet W. Smith and
- Horace D. Skipper
Vesicular-arbuscular mycorrhizal spores were extracted from soil (pot cultures) using adhesion-flotation, sucrose-centrifugation, gelatin column, wet-sieve, and a new plating method. Counts of 150- to 300-µm diam spores were significantly higher for the plate method by 528, 370, and 258% than for the adhesion-flotation, sucrose-centrifugation, and gelatin column methods, respectively. Counts of 100- to 300-µm diam spores were higher for the plate method by 200 and 149% than for the wet-sieve and modified wet-sieve methods. Spore counts within four spore size ranges (50 to 100, 100 to 150, 150 to 300, and > 300 µm diam) were greater for the plate method than for the wet-sieve and modified wet-sieve methods by approximately four and two times, respectively. The plate method was consistently higher than the wet-sieve method only for small spores (50 to 100 µm diam) from soybean field soils. Although the new plate method is not applicable for extracting and quantifying large spores (> 100 µm diam) for low spore population soils (field soils), it is recommended for the purpose of quantifying spores in high population soils (> 20 spores/g) as might be encountered in pot cultures.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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