Microbial Epimerization of Myo-inositol to Chiro-inositol in Soil1
- Michael F. L'Annunziata,
- Juan Gonzalez I. and
- Luis A. Olivares O.2
14Carbon-(1,2,3,5/4,6)-inositol (14C-myo-inositol) was incorporated into an Andosol sandy loam soil and incubated for a 12-day period at 70% field moisture capacity under sterile and nonsterile soil conditions. Comparison by thin-layer chromatography of an isolated metabolite with an authentic standard and infrared spectrometry were used to identify the metabolite as 14C-(1,2,4/3,5,6)-inositol (14C-chiroinositol) of unknown optical activity. The 14C-chiro-inositol was identified in the biologically active soil. No epimerization of 14C-chiroinositol was observed in sterile soil. The abundance of the 14C-chiroinositol was approximately 4% relative to that of the parent compound, as determined by liquid scintillation counting of the two chromatographically separated compounds.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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