Arylsulfatase Activity of Soils
- M. A. Tabatabai and
- J. M. Bremner
The detection of arylsulfatases (enzymes that hydrolyze aryl sulfates) in soils is reported, and a simple method of assaying soil arylsulfatase activity is described. This method involves colorimetric estimation of the p-nitrophenol released by arylsulfatase activity when soil is incubated with buffered (pH 5.8) potassium p-nitrophenyl sulfate solution and toluene at 37C for 1 hour, and it is rapid, sensitive, and precise. The procedure used to extract p-nitrophenol stops arylsulfatase activity, develops the yellow color used to estimate p-nitrophenol, and gives quantitative recovery of this compound. Studies of the properties of the arylsulfatases in soils are reported.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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