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This article in SSSAJ

  1. Vol. 34 No. 1, p. 70-72
     
    Received: June 24, 1969
    Published: Jan, 1970


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doi:10.2136/sssaj1970.03615995003400010021x

Michaelis Constant of Soil Urease1

  1. K. N. Paulson and
  2. L. T. Kurtz2

Abstract

Abstract

Application of the Michaelis-Menten equation to urease activity of soil showed that the Michaelis constant (Km) changed with fluctuation in microbial population and thus with changes in the proportion of microbial and adsorbed urease. Regression of urease activity on number of ureolytic microorganisms permitted division of total urease activity into portions caused by microbial and adsorbed forms, respectively. Computations using the partitioned urease activities gave Km's for the microbial and adsorbed forms of 0.057 and 0.252M, respectively. These values are similar in order of magnitude to those reported in the literature and thus further support the hypothesis that urease is active in both microbial and adsorbed forms in soil.

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