My Account: Log In | Join | Renew
Search
Author
Title
Vol.
Issue
Year
1st Page

Abstract

 

This article in SSSAJ

  1. Vol. 31 No. 3, p. 317-321
     
    Received: Oct 17, 1966
    Published: May, 1967


 View
 Download
 Alerts
 Permissions
 Share

doi:10.2136/sssaj1967.03615995003100030011x

Determination and Isotope-Ratio Analysis of Different Forms of Nitrogen in Soils: 7. Urea1

  1. D. R. Keeney and
  2. J. M. Bremner2

Abstract

Abstract

Two methods of determining urea in soils are described. In one (extraction-distillation method), the soil sample is extracted with 2M KCl (10 ml/g of soil) for 5 min, and an aliquot of the extract is treated with potassium phosphate buffer (pH 8.0) and urease for 2 hours at 30C to convert urea N to ammonium N. In the other (direct-distillation method), the soil sample is treated directly with pH 8.0 potassium phosphate buffer and urease for 1 hour at 30C. In both, the ammonium released by the urease treatment is separated by steam distillation of the treated sample for 3.3 min and determined by titration of the distillate with standard acid. The extraction-distillation method is useful when analyses for nitrate and nitrite as well as urea are required, but it does not give completely quantitative recovery of urea added to some soils. The methods are rapid, specific and precise, and they permit isotope-ratio analysis of urea N in N15-tracer studies of urea transformations in soils.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © . Soil Science Society of America