Isolation and Identification of Some Amino Compounds in Soils
- F. J. Stevenson
An isolation experiment was conducted to obtain sufficient amounts of all of the amino acids in a soil hydrolysate to permit their identification. This was done by a selective separation on a large column of Dowex 50, in which elution was carried out by hydrochloric acid. The identification of the various compounds was accomplished by the application of specific tests, by paper partition chromatography, and by ion exchange chromatography on a sodium column of Dowex 50, using the procedure of Moore and Stein.
Twenty-nine ninhydrin-reacting compounds were identified positively as products of acid hydrolysis of soils, one compound was identified tentatively, and three others were isolated but not characterized. In addition to the compounds previously verified, the following were confirmed: cysteic acid, N-acetyl-D-glucosamine, methionine sulfone, α, ε-diaminopimelic acid, α-amino-n-butyric acid, methionine sulfoxide, methionine, cystine, and ornithine. A discussion is given concerning the natural occurrence of these compounds in soils.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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