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This article in SSSAJ

  1. Vol. 16 No. 2, p. 170-172
     
    Published: Apr, 1952


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doi:10.2136/sssaj1952.03615995001600020016x

Chromatographic Separations of Inositol Phosphorus Compounds1

  1. Donald H. Smith and
  2. Francis E. Clark

Abstract

Abstract

A chromatographic technique for the separation of sodium phytate and its enzymatically produced derivatives is described. From a mixture of sodium phytate and derivatives, nine organic phosphorus compounds were separated in sufficient quantities to permit determination of their inositol/phosphorus ratios. Phosphorus was determined colorimetrically following perchloric acid digestion, and inositol was determined by means of a biological assay. The determinations were replicated sufficiently that the number of phosphorus atoms per molecule of inositol was considered to have been determined to within one atom of the true value in all cases. The identity of the nine separated compounds was tentatively established.

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