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This article in JEQ

  1. Vol. 22 No. 2, p. 375-378
     
    Received: May 26, 1992
    Published: Apr, 1993


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doi:10.2134/jeq1993.00472425002200020019x

Oxidation and Acidification of Anaerobic Sediment-Water Systems by Autoclaving

  1. Paul G. Tratnyek * and
  2. N. Lee Wolfe
  1. Dep. of Environ. Science and Eng., Oregon Graduate Inst. of Science and Technol., 19600 N.W. von Neumann Dr., Beaverton, OR 97006-1999;
    Environ. Res. Lab., USEPA, Athens, GA 30613-7799.

Abstract

Abstract

The effect of autoclaving on the acid/base and redox properties of anaerobic sediment slurries was determined to facilitate the interpretation of autoclaved controls in studies of environmental reduction reactions. Autoclaving decreased electrode measurements of pH by 0.26 to 0.68 units and increased electrode measurements of Eh by 34 to 94 mV. To corroborate this effect, we added redox indicators to the slurries and observed that autoclaving caused a shift in color consistent with the change in electrode measurements. Further investigation demonstrated that the observed effects are a common characteristic of autoclaving anaerobic sediment and are not limited to very restricted sediment types and are not artifacts of the way the experiments were performed.

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