Detection of PLDα1 alleles by cleaved amplified polymorphic sequence (CAPS) analysis. CAPS analysis of the PLDα1 alleles of ‘Koshihikari’, ‘03-s108’, and 32 F2 plants derived from the cross Koshihikari × 03-s108. The band sizes corresponding to the 03-s108 allele are 177, 145, 114, 82, and 32 bp; the band size of the Koshihikari allele is 259 bp. The 32-bp fragment is below the area shown in the figure. The PLDα1 alleles in each plant are shown below the photographs as + (homozygous for PLDα1-1), − (homozygous for pldα1-1), and ± (heterozygous).