Figure 1.

Schematic representation of the gene structure of rice PLDα1. Black boxes denote open reading frames, and the surrounding lines denote noncoding sequences. DNA polymorphisms found in 03-s108 are shown as vertical lines beneath the map; designations to the left and right of the arrows indicate sequence and amino acid from ‘Nipponbare’ and ‘03-s108’, respectively. Lines near the bottom of the figure (regions PLD1-1, PLD1-2, PLD1-3, and PLD1-SNP) represent the four DNA fragments amplified by polymerase chain reaction (see Table 1).

 


Figure 2.

Detection of PLDα1 alleles by cleaved amplified polymorphic sequence (CAPS) analysis. CAPS analysis of the PLDα1 alleles of ‘Koshihikari’, ‘03-s108’, and 32 F2 plants derived from the cross Koshihikari × 03-s108. The band sizes corresponding to the 03-s108 allele are 177, 145, 114, 82, and 32 bp; the band size of the Koshihikari allele is 259 bp. The 32-bp fragment is below the area shown in the figure. The PLDα1 alleles in each plant are shown below the photographs as + (homozygous for PLDα1-1), − (homozygous for pldα1-1), and ± (heterozygous).

 


Figure 3.

Detection of PLDα1 alleles by dot-blot-SNP analysis. Corresponding dots on each membrane represent the same F2 plant from the cross ‘Koshihikari’ × ‘03-s108’. As controls, the polymerase chain reaction products of ‘Nipponbare’ and 03-s108 DNA are blotted on each membrane (Nipponbare: positions B1 and B5; 03-s108: positions A1 and A5). The photographs show hybridization with a labeled PLD-NB probe and an unlabeled PLD-s108 probe (left), and with a labeled PLD-s108 probe and an unlabeled PLD-NB probe (right).