View Full Table | Close Full ViewTable 1.

Wildrye populations, barley cultivars, and genotypes (in parenthesis), along with their origins and seed sources examined for genetic diversity. Endophyte status of populations was determined using immunoblot and polymerase chain reaction (PCR) techniques.

 
Endophyte status Closest nonhybrid ancestor
Population (genotypes) Status Origin Seed source Immunoblot PCR seed PCR blade Species
Canada wildrye
Sharp's (Sh 143, 145, 147, 149, 150) Commercial source population Missouri Sharp Bros. Seed Co. + + Epichloë amarillans Eam
Stock's (St 154, 155, 156, 157, 159) Commercial source population Iowa Stock Seed Farms −/+ + + ND
98CWR8 (CW 1, 2, 3, 5, 7) Wild population North-central Texas NF collection + + + Neotyphodium sp. Eam × Eel
99CWR6 (CW 11, 12, 14, 16, 18) Wild population Central Texas NF collection + + + Neotyphodium sp. Eam × Eel
99CWR7 (CW 21, 24, 25, 27, 29) Wild population Central Texas NF collection -/+ + + Epichloë amarillans Eam
PI 436918 (CW 31, 33, 34, 36, 38) PI West Texas NPGS
PI 436933 (CW 41, 43, 45, 47, 49) PI Central Texas NPGS
PI 613134 (CW 51, 52, 54, 55, 56) PI Southeast Texas NPGS + + Epichloë elymi Eel
PI 436924 (CW 62, 63, 65, 67, 70) PI North-central Oklahoma NPGS ?
Virginia wildrye
03VWR2 (VW 71, 73, 74, 76, 78) Wild population South-central Oklahoma NF collection ND + Epichloë elymi Eel
PI 436945 (VW 83, 84, 85, 89, 90) PI Northeast Texas NPGS
PI 436955 (VW 93, 94, 96, 99, 100) PI Central Texas NPGS −/+
PI 436962 (VW 102, 103, 104, 105, 106) PI East-central Texas NPGS
PI 436968 (VW 113, 114, 115, 117, 118) PI Southeast Texas NPGS ND
Barley
Steptoe (S 122, 123) Cultivar Washington NPGS ND
Morex (M 132, 133) Cultivar Minnesota NPGS ND
NPGS = USDA National Plant Germplasm System.
NF = Noble Foundation.
§ + = endophyte infected; − = endophyte free.
Eam = Epichloë amarillans; Eel = Epichloë elymi
# ND, not determined.



View Full Table | Close Full ViewTable 2.

Number of microsatellite fragments obtained in genotypes of Canada wildrye and Virginia wildrye populations and barley cultivars from 38 tall fescue expressed sequence tag–simple sequence repeats.

 
Population Min. Max. Avg.
98CWR8 68 76 71.8
99CWR6 73 79 76.4
99CWR7 70 73 70.8
PI 613134 64 75 70.6
Sharp's 74 81 77.2
Stock's 66 75 70.7
PI 436918 63 70 67.2
PI 436924 65 71 68.4
PI 436933 66 72 68.8
CWR 63 81 71.3
03VWR2 60 71 68.0
PI 436945 64 72 66.8
PI 436955 60 75 67.4
PI 436962 68 71 69.0
PI 436968 66 75 70.4
VWR 60 75 68.3
Steptoe 45 50 47.5
Morex 51 52 51.5
Barley 45 52 49.5
SE 0.86 0.98 0.90