Figure 1.

An unrooted plot of consensus trees with a frequency greater than 50% after bootstrap analysis with 1000 replications. Bootstrap values are indicated on the trees. Genotypes represent 46 Canada wildrye (CW), 25 Virginia wildrye (VW), and four barley genotypes. PAUP* 4.0 beta was used to run the Neighbor joining algorithm. Genotypes correspond to the following populations: SH 143–150 = Sharp's; ST 154–159 = Stock's; CW 1–7 = 98CWR8; CW 11–18 = 99CWR6; CW 21–29 = 99CWR7; CW 31–38 = PI 436918; CW 41–49 = PI 436933; CW 51–56 = PI 613134; CW 62–70 = PI 436924; VW 71–78 = 03VWR2; VW 83–90 = PI 436945; VW 93–100 = PI 436955; VW 102–106 = PI 436962; VW 113–118 = PI 436968; S 122–123 = ‘Steptoe’ barley; M 132–133 = ‘Morex’ barley. E+ indicates populations that were endophyte infected based on polymerase chain reaction analysis.

 


Figure 2.

Sequences of fragments amplified by tall fescue expressed sequence tag–simple sequence repeat primer NFFA113 in barley cultivars and Canada wildrye and Virginia wildrye populations. Primer sequences are italicized, base substitutions are bold faced, and repeat sequences are in bold italics. The sequences represent a single genotype from each of the cultivars/populations.

 


Figure 3.

Endophyte detection in seeds and leaf blades of Canada wildrye and Virginia wildrye populations. Twelve individual seeds from each population and DNA from leaf blades of each plant were screened for the presence of the tef1 gene from epichloë endophytes using endophyte-specific polymerase chain reaction primers.