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This article in CS

  1. Vol. 8 No. 4, p. 506-507
     
    Received: Feb 23, 1968
    Published: July, 1968


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doi:10.2135/cropsci1968.0011183X000800040034x

Germination of Oat (Avena Byzantina C. Koch) Pollen on Artificial Media1

  1. A. T. Wallace and
  2. P. Karbassi2

Abstract

Abstract

Freshly collected oat pollen was germinated in Petri dishes on media containing 13 g sucrose, 1.7 g raffinose, 20 mg boric acid, 25 mg calcium nitrate, 3 g bacto-agar, and 85 ml double-distilled water. Germination is improved if the dishes containing the media are "aged" 2 to 10 days in a refrigerator. The pollen germinates within 1 to 2 minutes after it comes in contact with the media. Within another 5 to 6 minutes the tubes usually have grown to their maximum length, 125 to 150 microns, at which time the ends burst and exude their contents. Modifying agents proved to be ineffective in preventing the bursting.

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