A New Method of Using Interchanges as Chromosome Markers1
- C. R. Burnham2
A new method of utilizing interchanges as chromosome markers which will require one less than the n number of chromosomes to test the entire genome for linkage is proposed. The underlying principle is that each of the interchanged chromosomes in each stock would be marked by two widely separated breakpoints. Breeding procedures in maize for constructing a series with 2-chromosome, double interchange stocks are described and illustrated by the results from a cross between two genetically marked T1-5 interchange stocks. Breeding procedures for a series with 3-chromosome, triple interchange stocks are described also.
The detection of linkage with any major gene, using either the double or the triple interchange series should be much more efficient than tests using single interchanges. When heterozygous, many of the products of crossing over in the differential segments are deficient and hence not recoverable. Also only part of the recoverable crossovers are recognizable as recombinants.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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