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This article in CS

  1. Vol. 51 No. 4, p. 1832-1839
     
    Received: Oct 1, 2010
    Published: July, 2011


    * Corresponding author(s): john.grabber@ars.usda.gov
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doi:10.2135/cropsci2010.10.0566

Rumen-Degradable Protein in Roll-Conditioned or Macerated Legume Hays and Silages Estimated by In Situ Kinetics vs. Alternative Methods

  1. J. H. Grabber *a,
  2. W. K. Coblentzb and
  3. G. A. Brodericka
  1. a USDA-ARS, U.S. Dairy Forage Research Center, Madison, WI 53706
    b USDA-ARS, Institute for Environmentally Integrated Dairy Management, Marshfield, WI 54449

Abstract

Alternatives to the in situ method for estimating rumen-degradable protein (RDP) in diverse forage legumes should be validated. In this study, RDP in roll-conditioned or macerated silages and hays of Medicago, Lotus, and Trifolium species with differing polyphenol compositions were estimated from in situ degradation kinetics, a rumen microbial inhibitor in vitro assay, a 10-h in situ incubation, the Cornell Net Carbohydrate and Protein System, and a 16-h in vitro Streptomyces griseus protease digestion. Forage species, conditioning, and conservation methods influenced RDP estimates. In situ kinetic RDP could be ranked or in some cases accurately predicted by other RDP methods, but the protease method performed poorly for macerated forages.

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