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This article in CS

  1. Vol. 51 No. 4, p. 1491-1497
     
    Received: Dec 19, 2010
    Published: July, 2011


    * Corresponding author(s): ramsey_lewis@ncsu.edu
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doi:10.2135/cropsci2010.09.0546

Identification of Tobacco Haploids on the Basis of Transgenic Overexpression of PAP1 from Arabidopsis thaliana

  1. Ramsey S. Lewis * and
  2. Cara Rose
  1. Campus Box 7620, Crop Science Dep., North Carolina State Univ., Raleigh, NC 27695

Abstract

Haploid plants have utility in plant breeding for several purposes. In tobacco (Nicotiana tabacum L.) gynogenic haploids can be produced from seed due to parthenogenesis. For practical use, a system is needed to identify infrequent haploid plants at the seed or seedling stage. Interspecific hybridization with N. africana is presently used to isolate gynogenic tobacco haploids because a suitable dominant seedling marker does not currently exist for N. tabacum. Here we investigated the utility of a purple seedling trait conferred by overexpression of the Arabidopsis gene, PAP1, to identify gynogenic haploids produced from seed. Two tobacco cultivars were crossed as females with a genetic stock homozygous at two 35S:PAP1 transgene loci. Gynogenic haploids were recognized as green seedlings among purple F1 hybrid seedlings. The average frequency of gynogenic haploidy using this system was f = 0.00027. For comparison, the same cultivars were also hybridized as females with N. africana. The average frequency of gynogenic haploidy using this system was approximately seven times higher than that observed for the 35S:PAP1 system. Having a dominant seedling marker for N. tabacum may permit development of genetic stocks that contribute to an increased predisposition for haploid formation via parthenogenesis. In addition, the 35S:PAP1 genetic marker may have utility for identifying androgenic haploids from seed for the purpose of rapidly generating alloplasmic lines of tobacco.

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