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This article in CS

  1. Vol. 51 No. 1, p. 282-289
     
    Received: Mar 1, 2010
    Published: Jan, 2011


    * Corresponding author(s): Joseph.Burton@ars.usda.gov
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doi:10.2135/cropsci2010.03.0123

Inositol Metabolism in Developing Seed of Low and Normal Phytic Acid Soybean Lines

  1. Daniel W. Israela,
  2. Earl Talierciob,
  3. Prachuab Kwanyuenb,
  4. Joseph W. Burton *b and
  5. Lisa Deanc
  1. a USDA-ARS and Department of Soil Science, North Carolina State University, Raleigh, NC 27695
    b USDA-ARS, 3127 Ligon Street, Raleigh, NC, 27607
    c USDA-ARS, Food Bioprocessing & Nutrition Sciences, North Carolina State University, Box 7624, Raleigh, NC, 27695

Abstract

While inositol has key roles in phytic acid and raffinosaccharide synthesis, its concentration in developing seed of low phytic acid soybean [Glycine max (L.) Merr.] lines derived from CX1834 (Wilcox et al., 2000) has not been compared to that of normal lines. Concentrations of metabolites in the phytic acid and raffinosaccharide biosynthesis have been measured in mature seed of CX1834-derived lines but not throughout seed development. Our objective was to compare concentrations of inositol and metabolites associated with phytic acid and raffinosaccharide synthesis in developing seed of CX1834-derived and normal lines. Plants were cultured with complete nutrient solutions in growth chambers with 650 to 700 μmol m−2 s−1 of photosynthetically active radiation and a 26/22°C day/night temperature. Seed inositol concentrations were high (60 to 90 mmol kg−1 seed dry wt.) at 20 d after flowering (DAF) and decreased 95% by maturity in both normal and low phytic acid lines. In two of three experiments, low phytic acid lines had significantly (p ≤ 0.05) greater seed inositol concentrations than normal lines at the first two sampling dates, but differences at maturity were not significant. Seeds of low phytic acid and normal lines had statistically similar concentrations (p > 0.05) of partially phosphorylated inositol intermediate (inositol triphosphate [IP3]), stachyose, raffinose, and phytase activity throughout development. These results corroborate previous studies that ruled out defects in genes coding myo-inositol-1-P synthase, inositol kinases, and phytase as the basis for the low seed phytic acid trait in CX1834-derived lines.

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