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This article in CS

  1. Vol. 47 No. 2, p. 685-691
     
    Received: Aug 16, 2006
    Published: Mar, 2007


    * Corresponding author(s): gallais@moulon.inra.fr
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doi:10.2135/cropsci2006.08.0523

Estimating the Proportion of Nitrogen Remobilization and of Postsilking Nitrogen Uptake Allocated to Maize Kernels by Nitrogen-15 Labeling

  1. A. Gallais *a,
  2. M. Coquea,
  3. J. Le Gouisc,
  4. J. L. Priould,
  5. B. Hirelb and
  6. I. Quilléréb
  1. a Station de Génétique Végétale, INRA-UPS-INAPG-CNRS, Ferme du Moulon, 91190 Gif/Yvette, France
    c UMR INRA-USTL Stress abiotiques et différenciation des végétaux cultivés, Estrées-Mons, 80203 Péronne, France
    d Institut de Biotechnologie des Plantes, Université de Paris-Sud, 91405 Orsay Cedex, France
    b Unité de Nutrition Azotée des Plantes, INRA route de St Cyr, 78026 Versailles Cedex, France

Abstract

Estimating the proportion of N remobilization and postsilking N uptake allocated to kernels may help to improve N-use efficiency in maize (Zea mays L.). In this study, we show theoretically and experimentally, that 15N labeling at the beginning of stem elongation can be used in the field to estimate the proportion of N remobilized from the vegetative parts to the kernels of maize by measuring 15N distribution only at maturity. In the same way, 15N labeling at silking allows a determination of the proportion of postsilking N uptake allocated to the kernels by measuring 15N distribution only at maturity. Two 1-yr experiments with three and four genotypes and one 2-yr experiment with testcross progenies from 66 recombinant inbred lines were developed. Nitrogen-15 labeling during vegetative growth provided an estimate of the proportion of N remobilized with a greater accuracy compared with the “balance” method, which computes the difference between the amount of N present in the stover at silking and the amount of N present in the stover at maturity. The validity of our 15N method is mainly based on the assumption that less than 15 to 20% of 15N is taken up after silking. The determination of the proportion of postsilking N uptake allocated to kernels requires assumptions that are more difficult to fulfill. Nitrogen-15 labeling in the field at the beginning of rapid stem growth appears to be a useful tool for studying the genetic variability of N remobilization using a large number of genotypes.

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Copyright © 2007. Crop Science Society of AmericaCrop Science Society of America