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  1. Vol. 46 No. 1, p. 162-167
     
    Received: Apr 15, 2005
    Published: Jan, 2006


    * Corresponding author(s): sfei@iastate.edu
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doi:10.2135/cropsci2005.04-0008

Analysis of Genetic Diversity in Rough Bluegrass Determined by RAPD Markers

  1. Shanmugam Rajasekar,
  2. Shui-hang Fei * and
  3. Nick E. Christians
  1. Department of Horticulture, 257 Horticulture Hall, Iowa State University, Ames, IA 50011, USA

Abstract

Information on genetic variation in rough bluegrass (Poa trivialis L.), a cool-season grass that is grown for sports fields and lawns, is needed. The objective of this study was to assess genetic variation in 27 accessions of rough bluegrass obtained from the National Plant Germplasm System (NPGS) by random amplified polymorphic DNA (RAPD) markers. Fifteen of the 80 primers screened generated 64 highly repeatable polymorphic bands. A dendrogram constructed on the basis of the Unweighted Pair Group Method with Arithmetic Average (UPGMA) clustering algorithm revealed that 26 of 27 accessions formed two distinct clusters. Genetic similarity coefficients calculated from the RAPD data ranged from 0.07 to 0.74 with the lowest value of 0.07 measured between PI 254908, PI 594396, PI 250982, and PI 229782 from Iraq, the USA, Yugoslavia, and Iran, respectively. The highest value of 0.74 was measured between PI 225826, PI 289643, and PI 592521 from Denmark, Spain, and the USA, respectively. The cophenetic correlation coefficient (r) was 0.90, indicative of a very good fit between the data matrix and the resulting cluster analysis. Principal coordinate analysis (PCO) clearly grouped 26 accessions on the two axes, with a single accession that did not cluster with the others. The PCO clustering pattern corresponded well with the dendrogram. PI 254908 from Iraq did not cluster with any other accessions, either in the dendrogram or on the basis of the PCO.

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