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This article in CS

  1. Vol. 39 No. 2, p. 353-357
     
    Received: Feb 26, 1998
    Published: Mar, 1999


    * Corresponding author(s): zasto@ttacs.ttu.edu
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doi:10.2135/cropsci1999.0011183X003900020008x

Selection of Castor for Divergent Concentrations of Ricin and Ricinus communis Agglutinin

  1. Scott D. Pinkerton,
  2. Rial Rolfe,
  3. Dick L. Auld ,
  4. Victor Ghetie and
  5. Brenda F. Lauterbach
  1. Dep. of Plant and Soil Science, Texas Tech Univ., Lubbock, TX 79409-2122
    Microbiology and Immunology, 5B191 Health Science Center Building, Texas Tech Univ./Health Sciences Center, Lubbock, TX 79409
    Cancer Immunobiology Center and Dep. of Microbiology, U.T. Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235-8576

Abstract

Abstract

One of the most limiting factors in the production and processing of castor (Ricinus communis L.) is the presence of ricin and Ricinus communis agglutinin (RCA) in the endosperm. These highly toxic proteins create potential health hazards that have limited castor production in the USA, The objectives of this research were to develop a rapid assay to quantify ricin + RCA concentration in whole and partial seed of castor; to screen the USDA castor germplasm collection for ricin + RCA concentration; and to determine the inheritance of ricin + RCA in segregating populations of castor. A radial immunodiffusion (RID) assay based on ricin specific antibodies was shown to be highly repeatable in estimating the relative ricin + RCA concentrations of 18 castor accessions (rs = 0.98**). This assay could be used on whole seed or a small portion of the endosperm removed distal to the caruncle to maintain seed viability. The ricin + RCA concentration of 263 accessions from the USDA castor collections ranged from 1.9 to 16.0 mg g−1. Two accessions, PI 257 654 and PI 258 368, with low ricin + RCA concentrations were crossed with the commercial cultivar Hale. Three F3 seed from each F2 plant of six segregating populations were evaluated for ricin + RCA concentration. Individual plants of the six F2 populations and seed of the six F3 populations had an extremely wide range and significantly differed in ricin + RCA concentration. F3 plants with low and high levels of ricin + RCA were selected.

This is contribution number T-4-430 of the College of Agric. Sci. and Natural Resources, Texas Tech Univ.

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