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This article in CS

  1. Vol. 38 No. 1, p. 221-225
     
    Received: Dec 24, 1996
    Published: Jan, 1998


    * Corresponding author(s): geizenga@ag.gov
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doi:10.2135/cropsci1998.0011183X003800010037x

Differentiation of Tall Fescue Monosomic Lines Using RFLP Markers and Double Monosomic Analysis

  1. G. C. Eizenga ,
  2. C. L. Schardl,
  3. T. D. Phillips and
  4. D. A. Sleper
  1. U SDA-ARS, NRGEEC, Stuttgart, AR 72160
    D ep. of Plant Pathology, Univ. of Kentucky, Lexington, KY 40546
    D ep. of Agronomy, Univ. of Kentucky, Lexington, KY 40546
    D ep. of Agronomy, Univ. of Missouri, Columbia, MO 65211

Abstract

Abstract

Tall fescue (Festuca arundinacea Schreb., 2n =6x =42) is a major cool-season pasture and turf grass with the genome constitution PPG1G1G2G2. Lack of aneuploid stocks hinders genetic studies in this important grass. The objective of this study was to characterize 23 fertile, embryo culture-derived, ‘Kenwell’ tall fescue monosomic lines by means of (i) RFLP markers, (ii) double monosomic analysis, and (iii) isozyme phenotypes of F1 progeny. Standard procedures were used for RFLP chemiluminescent detection, crossing, cytogenetic analyses, and obtaining isozyme banding patterns. Using RFLP markers, we ascertained that four monosomic lines were hemizygous for a P-genome marker and the 23 plants were placed into 17 groups based on plants having the same RFLP banding patterns. For double monosomic analysis, metaphase I chromosome pairing relationships were ascertained for 220 F1 progeny from 26 crosses among the monosomic lines. Five of the progeny were trisomic, 148 disomic, 60 monosomic, and seven had 40 chromosomes. All of the 40-chromosome progeny were double monosomic plants, indicating the monosome carried in the parents were not the same chromosome. Isozyme phenotypes were ascertained for 203 F1 progeny from crosses for double monosomic analysis which had both 42- and 41-chromosome progeny to determine if an isozyme locus mapped to the monosome from the female parent. A PGI-2 locus most likely mapped to one monosome. Double monosomic analysis provided evidence that at least four of the 21 possible tall fescue monosomic lines were obtained.

Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA or the Univ. of Kentucky and does not imply its approval to the exclusion of other products that may also be suitable. Contribution as Journal Paper No. 96-06-192 of the Kentucky Agric. Exp. Stn.

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