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This article in CS

  1. Vol. 36 No. 6, p. 1709-1711
     
    Received: Jan 23, 1996
    Published: Nov, 1996


    * Corresponding author(s): congerbv@utk.edu
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doi:10.2135/cropsci1996.0011183X003600060049x

Micropropagation of Switchgrass by Node Culture

  1. K. S. Alexandrova,
  2. P. D. Denchev and
  3. B. V. Conger 
  1. The University of Tennessee, Dep. of Plant and Soil Science, Knoxville, TN 37901-1071

Abstract

Abstract

Switchgrass (Panicum virgatum L.) is naturally outcrossing; therefore, maintenance of genotype is difficult through sexual propagation. The objective of this study was to develop a micropropagation procedure for the multiplication of desired or selected genotypes. Nodal segments were surface sterilized, split longitudinally, and placed with the cut surface on solid MS medium containing 30 g L−1 maltose and different concentrations of 6-benzylaminopurine (BAP) as the only growth regulator. Best shoot proliferation was obtained with 12.5 μM BAP and culture at 29°C. Shoots were easily rooted on MS medium without BAP. Under optimum conditions, it is possible to produce approximately 500 plantlets from one parent plant in 12 wk.

Research was supported in part by Lockheed Martin Energy Systems Inc. under Subcontract No. 11X-SL129C.

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