Comparison of DNA Marker Technologies in Characterizing Plant Genome Diversity: Variability in Chinese Sorghums
- Wenpeng Yang,
- Antonio C. de Oliveira,
- Ian Godwin,
- Keith Schertz and
- Jeffrey L. Bennetzen
- D ep. of Biological Sciences, Purdue Univ., West Lafayette, IN 47907-1392 and Institute of Upland Food Crops, Guiyang, Guizhou 550006, PRC
D ep. of Biological Sciences, Purdue Univ., West Lafayette, IN 47907-1392
D ep. of Biological Sciences, Purdue Univ., West Lafayette, IN 47907-1392 and Dep. of Agriculture, The University of Queensland, Brisbane Qld 4072, Australia
U SDA-ARS and Dep. of Soil and Crop Sciences, Texas A & M University, College Station, TX 77843
Chinese sorghums [Sorghum bicolor (L.) Moench] are reputed to have a narrow genetic base, resulting from infrequent introduction of exotic germplasm into China. We have used several different molecular approaches to evaluate diversity and relatedness in a selection of 34 Chinese sorghums. The results indicated that different DNA marker technologies for germplasm assessment yield comparable results, but that a relatively new technique termed inter-simple sequence repeat amplification (ISSR) was relatively rapid, reproducible, and inexpensive. Extensive diversity was observed within the Chinese sorghums, and all lines could be easily differentiated. Different lines collected from the same locality were found to exhibit particularly high levels of relatedness. Contrary to expectations, improved varieties were found to contain more diversity and to be more different from each other than were the Chinese landraces studied, suggesting recent introduction of non-Chinese germplasm into these improved materials.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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