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This article in CS

  1. Vol. 33 No. 6, p. 1397-1400
     
    Received: Nov 23, 1992
    Published: Nov, 1993


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doi:10.2135/cropsci1993.0011183X003300060053x

Dehydration Stress-Induced Changes in Translatable Rnas in Sorghum

  1. Y. Cheng,
  2. J. Weng,
  3. C. P. Joshi and
  4. H. T. Nguyen 
  1. Plant Molecular Genetics Lab., Mail Stop 2122, Dep. of Agronomy, Horticulture, and Entomology, Texas Tech Univ., Lubbock, TX 79409

Abstract

Abstract

Sorghum [Sorghum bicolor (L.) Moench] is a drought-resistant crop species. The main objective of this work was to study the dehydration stress-induced changes in mRNA accumulation in sorghum. Ten-day old seedlings were dried in a closed chamber for 6, 10, 14, 18, and 22 h. Poly(A)+ RNAs, isolated from control and dehydration-stressed shoots, were translated in vitro in the presence of 35S methionine and the translation products were separated by two-dimensional polyacrylamide gel electrophoresis. Four types of polypeptides which exhibited significant changes during the time course were distinguished. Northern blot analysis using a eDNA encoding a dehydration-induced protein (dehydrin) revealed a rapid induction and increased accumulation of dehydrin mRNA species throughout the drought stress process. These results suggest that sorghum seedlings, similar to other plant species, exhibit altered gene expression patterns in response to the length of drought stress period.

Contribution of the College of Agric. Sci., Texas Tech Univ. Journal no. (T-4-355). This work was supported by the Texas Tech. Univ. and Inst. for Biotechnol.

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