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This article in CS

  1. Vol. 32 No. 6, p. 1502-1505
     
    Received: Nov 12, 1991
    Published: Nov, 1992


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doi:10.2135/cropsci1992.0011183X003200060039x

Nulls for the Major Soybean Bowman-Birk Protease Inhibitor in the Genus Glycine

  1. Juliann M. Domagalski,
  2. Krishna P. Kollipara,
  3. Anne H. Bates,
  4. David L. Brandon,
  5. Mendel Friedman and
  6. Theodore Hymowitz 
  1. Dep. of Agronomy, 1102 S. Goodwin Ave., Univ. of Illinois, Urbana, IL 61801

Abstract

Abstract

The Bowman-Bilk protease inhibitor (BBI) is a major antinutritional factor in soybean [Glycine max (L.) Merr.]. This study was conducted to determine nulls for the major soybean BBI in the genus Glycine. A monoclonal antibody-based competitive enzyme-linked immunosorbent assay (ELISA) was used to rapidly detect the relative levels of the major soybean BBI in unpurified seed extracts. A total of 12 690 accessions from 13 species were tested. All of the G. max and G. soja Siebold & Zucc. accessions from the USDA Soybean Germplasm Collection contained BBI. The BBI nulls were found hi several of the wild perennial species: G. curvata Tind., G. cyrtoloba Tind., G. latifolia (Benth.) Newell & Hymowitz, G. microphylla (Benth.) Tind, G. tabacina (Labill.) Benth., and G. tomentella Hayata. The absence of BBI was confirmed by additional immunochemical methods and by enzymatic determination of trypsin and chymotrypsin inhibition. The BBI nulls identified in this study could potentially be utilized in the production of a soybean cultivar lacking the major soybean BBI.

Research supported in part by the Illinois Agric. Exp. Stn., USDA Res. Agreement no. 58-91H2-M-239, and a grant to T. Hymowitz from the Illinois Soybean Program Operating Board (88-14-87-3).

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