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  1. Vol. 30 No. 4, p. 950-952
     
    Published: July, 1990


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doi:10.2135/cropsci1990.0011183X003000040040x

Linolenic Acid Content of Soybean Seed Estimated with 2-Thiobarbituric Acid Test

  1. D. M. Bubeck,
  2. D. N. Duvick,
  3. W. R. Fehr  and
  4. E. G. Hammond
  1. Dep. of Agronomy
    Dep. of Food Technology, Iowa State Univ., Ames, IA 50011

Abstract

Abstract

The linolenic acid content in soybean [Glycine max (L.) Merr.] seed oil generally is determined by gas chromatography (GC). A more rapid method of comparing the linolenic acid content of soybean seed samples would be useful for screening a large number of lines in a soybean breeding program. The objective of this study was to determine if the 2-thiobarbituric acid (TBA) reaction could be used as a rapid method for determining the relative differences in linolenic acid content of soybean seed samples. Sixty-eight random F2 plants from each of two populations were used to compare TBA scores with linolenic acid content as measured by GC. The correlations between TBA score and linolenic acid percentages were 0.71 for Cross 1 and 0.60 for Cross 2. The results indicated that the TBA test cannot be used as a substitute for GC analysis in evaluating soybean samples. However, the TBA test may be a useful method to rapidly eliminate genotypes with excessively high linolenic acid before GC analysis.

Contribution from the Texas Agric. Exp. Stn., Journal Article TA24877. Funded in part by a grant from the U.S. Golf Assoc. Green Section.

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