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  1. Vol. 30 No. 3, p. 571-575
     
    Received: July 17, 1989
    Published: May, 1990


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doi:10.2135/cropsci1990.0011183X003000030021x

Gene Transfer in Apomictic Buffelgrass through Fertilization of an Unreduced Egg

  1. E. C. Bashaw  and
  2. K. W. Hignight
  1. USDA-ARS Southern Crops Res. Lab. and Soil and Crop Sciences Dep., Texas A&M Univ., College Station, TX 77843

Abstract

Abstract

Opportunities for gene manipulation are often limited in apomictic species, and conventional breeding methods may be totally ineffective. Lack of good sexual germplasm led us to investigate improvement of apomictic buffelgrass (Cenchrus ciliaris L.) through fertilization of an unreduced egg (2n+n), using an apomict as the female parent. The primary objective of this research was to determine the potential for gene transfer in buffelgrass through 2n+n hybridization. When an apomictic pentaploid accession with an alien genome (2n=5x=45) was crossed with tetraploid apomictic birdwoodgrass (C. setigerus Vahl, 2n=4x=36), 1.3% of 950 progeny resulted from the fertilization of an unreduced egg (BIII hybrids) and had 2n = 7x=63 chromosomes. Origin of these hybrids from the union of 2n and n gametes was confirmed by the presence of the alien genome from the female parent and diagnostic morphological characteristics of the male parent. Hybrids were all obligate apomicts and most were relatively high in seed set compared to the parents. The results demonstrated an effective method for gene transfer in some apomictic species. Complete emasculation was considered essential for maximum hybridization.

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Copyright © 1990. Crop Science Society of AmericaCopyright © 1990 by the Crop Science Society of America, Inc.