In Vitro Plant Regenation of Peanut From Seed Explants
- A. H. McKently *,
- G. A. Moore and
- F. P. Gardner
An efficient system of plant regeneration is critical to the success of newly developing genetic manipulation techniques as tools for crop improvement programs. This study was designed to assess the regeneration response of explants taken from the seed of peanut (Arachis hypogaea L.). Complete plants were regenerated from in vitro-cultured embryo axes and embryonated and deembryonated cotyledons that were whole and sectioned. Multiple shoots arose on 6-benzylaminopurine (BA) supplemented Murashige and Skoog media (0.5–60 mg L-1), with maximum production occurring at 25 mg L-1. Large quantities of shoots were obtained from single whole embryonated cotyledon explants cultured on BA-supplemented medium for periods up to 238 d. Excised shoots developed roots in vitro upon transfer to medium supplemented with 1-napthaleneacetic acid (NAA) at 1 mg L-1 for 30 d. In vitro-produced plantlets transferred to soil and placed in a greenhouse developed successfully, matured, and set seed. Twenty genotypes were regenerated using this tissue culture system. No phenotypic variants were observed among any of the plants produced in these experiments. Thus, this system allows for the in vitro production of morphologically normal peanut plants at high frequencies.
Copyright © 1990 by the Crop Science Society of America, Inc.