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  1. Vol. 28 No. 1, p. 157-162
     
    Received: Jan 28, 1987
    Published: Jan, 1988


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doi:10.2135/cropsci1988.0011183X002800010034x

Use of Near Infrared Reflectance Spectroscoppy to Screen Soybean Lines for Plant Nitrogen

  1. H. H. Mündel  and
  2. G. B. Schaalje
  1. Agric. Canada Res. Stn., Lethbridge, Alberta, Canada T1J 4B1

Abstract

Abstract

Selecting breeding lines of soybean [Gfycine Max (L.) Merr.] for an enhanced ability to support N fixation by Bradyrhizobium japonicum Jordan requires a fast screening method for determining N content in the plants. The objective of this study was to determine if near infrared spectroscopy (NIRS) could be used as such a method. Two cultivars grown at two locations with 10 inoculation treatments were used for field samples. One cultivar grown at two temperature regimes with 40 N fertilizer treatments was used for the phytotron samples. Samples were ground and regressions were run of log (I/relectance) of up to 19 filters (wavelengths) on a Technicon InfraAlyzer 400 (Technicon Instruments Corp., Tarrytown, NY) to laboratory Kjeldahl N determinations. Filters were selected to optimize regressions; overall stage and/or part regressions as well as individual stage and/or part (R2, R8—overall top growth, R8—seeds, and R8—top growth excluding seeds) regressions were carried out. Tests of homogeneity of regressions showed that stages and/or parts could not be combined, but that field and phytotron-grown soybean could be combined. Predictions run on random samples were most accurate for N content in seeds and at R2 and least accurate for R8—top growth excluding seeds. Calibrations based on field and phytotron samples from 1 yr were acceptable for predicting N content in a subsequent year. The NIRS calibrations were on the average more highly repeatable for predicting N content than either one of two Kjeldahl determinations.

Contribution from Agric. Canada Res. Stn.

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