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This article in CS

  1. Vol. 26 No. 5, p. 1072-1074
     
    Received: Oct 25, 1985
    Published: Sept, 1986


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doi:10.2135/cropsci1986.0011183X002600050048x

Anther Culture of Elite Genotypes of Maize1

  1. J. F. Petolino and
  2. A. M. Jones2

Abstract

Abstract

The use of anther culture as a means of haploid breeding in maize (Zea mays L.) has been limited by the paucity of responsive genotypes. The present study was designed to identify agronomically acceptable germplasm of maize that responds to anther culture. In 1984, 25 related-line crosses were evaluated for their ability to produce embryoids from cultured anthers. Twelve genotypes responded with at least one anther producing embryoids; with six exhibiting response frequencies of 1.0% or greater. In 1985, 27 genotypes were screened and 17 responded, nine of which displayed frequencies of 1.0% or greater. The most responsive crosses contained the lines H99, FR16, and Pa91. Plants could be regenerated from most responsive genotypes, however, seed production resulting from spontaneous chromosome doubling was low.

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