Genetic Analysis of a Null-Allele for Lipoxygenase-2 in Soybean1
- C. S. Davies and
- N. C. Nielsen2
Soybean [Glycine max (L.) Merr.] seeds contain at least three isomeric forms of lipoxygenase. We have identified a soybean line, PI 86.023, which is homozygous for a gene that confers the absence of the lipoxygenase-2 isozyme. Analysis of electrophoretic banding patterns of seed extracts from the F2 and F3 progeny of crosses between PI 86.023 and breeding lines containing null-alleles for lipoxygenase- 1 and -3 indicated that this gene was tightly linked to the Lx1 locus and independent of the Lx3 locus. We propose that the dominant allele for this gene be called Lx2 and the recessive null be designated lx2. Seeds were identified that lacked both lipoxygenase-2 and -3, but none was found that lacked both lipoxygenase-1 and -2. The lx-2 null-allele will be useful for investigating the effect of lipoxygenase-2 on the production of off-flavors during processing of soybean products.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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