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  1. Vol. 22 No. 4, p. 716-719
     
    Received: Feb 3, 1981
    Published: July, 1982


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doi:10.2135/cropsci1982.0011183X002200040005x

Variation among Inbred Lines of Crimson Clover for N2 Fixation (C2H2) Efficiency1

  1. G. R. Smith,
  2. W. E. Knight and
  3. H. H. Peterson2

Abstract

Abstract

Inbred lines of crimson clover (Trifolium incarnatum L.) were assayed for N2 fixation using the acetylene reduction assay. The seedlings were inoculated with a single pure strain (162K13) of Rhizobium trifolii and cultured in a nil-nitrogen nutrient solution contained in individual plastic growth pouches. The acetylene reduction assay was carried out on live plants using the plastic pouches to contain the gas mixture. In the first experiment, significant differences were noted among 14 crimson clover lines for acetylene reduction. Top dry matter, leaf number, and visual scores of nodulation, root growth and top growth were significantly correlated with the acetylene reduction assay. A relatively large coefficient of variation (27.1%) was noted for this experiment. In the second experiment refinement of culture and assay techniques resulted in a lower coefficient of variation (15.2%) and significant differences among six crimson clover lines for acetylene reduction were found.

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