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This article in CS

  1. Vol. 16 No. 5, p. 731-732
     
    Received: Apr 13, 1975
    Published: Sept, 1976


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doi:10.2135/cropsci1976.0011183X001600050035x

Ethidium Bromide Induced Cytoplasmic Male Sterility in Pearl Millet1

  1. Glenn W. Burton and
  2. Wayne W. Hanna2

Abstract

Abstract

Seeds of homozygous ‘Tift 23DB1’ pearl millet [Pennisetum americanum (L.) K. Schum], maintainer for cytoplasmic male sterile (cms) ‘Tift 23DA1,’ were soaked in deionized water solutions of 250 and 1,000 ppm of ethidium bromide (EB) at 5 C for 40 hours. Treated seeds were rinsed in tap water for 30 min, surface-dried, and planted in replicated two-row plots in the field with two-row plots of untreated Tift 23DB1. Male-sterile mutants (whole heads, sectored heads, or heads with intermingled male-sterile and male-fertile florets) occurred at frequencies of 1 in 30,100, I in 515, and 1 in 274 in total head populations of 30,100, 22,700, and 19,200 treated with 0, 250, and 1,000 ppm EB, respectively. Occurrence of M1 sterile mutants that set seed when pollinated and gave rise to some cms progenies in the greenhouse proved that the mutants were cytoplasmic male sterile.

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