Selection of High Lysine Corn Using a Scanning Electron Microscope1
- C. E. Wassom and
- R. C. Hoseney2
Several inbred sources of corn (Zea mays L.), including high-lysine modified opaque-2 lines with hard, translucent endosperm and normal hard, translucent endosperm phenotypes were examined with a scanning electron microscope. Zein bodies were observed in the protein matrix of the normal corn phenotype. Placing samples in 70% ethanol and observing voids produced confirmed that the bodies contained zein. Samples from modified opaque2 lines known to be high in lysine did not show elaborate patterns of voids in the rotein matrix after ethanol treatment. It was conclufed that a scanning electron microscope is an excellent tool to identify endosperms with low zein and potentially more lysine.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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