Oxidation and Phosphorylation By Mitochondria Isolated From Iron-sufficient and Iron-deficient Corn Roots
- Helen H. Young and
- A. Wallace1
Active mitochondria were successfully isolated from roots of Fe-deficient and Fe-sufficient corn (Zea mays L.) plants. Citrate, DL-isocitrate, α-ketoglutarate, succinate, L-malate, and NADH were oxidized by the mitochondria from either kind of corn plant. Fe-deficient root mitochondria had decreased O2 uptake and oxidative phosphorylation as compared to those of Fe-sufficient root mitochondria. Furthermore, mitochondrla from Fe-deficient corn roots had a low respiratory control ratio and the O2 uptake had little response to the addition of ADP. Measurements of NADH oxidation by a spectrophotometric method showed that Fe-deficient root mitochondria reduced the affinity between substrate and the nonheme Fe enzyme (NADH dehydrogenase).Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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