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This article in AJ

  1. Vol. 75 No. 6, p. 1049-1050
     
    Received: Feb 14, 1983
    Published: Nov, 1983


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doi:10.2134/agronj1983.00021962007500060041x

A Reliable Method for Processing Soybean Seed for Laboratory Analysis1

  1. J. A. Schild and
  2. R.A. Olson2

Abstract

Abstract

Established procedures for milling soybean [Glycine max (L.) Merr.] seed entail use of solvents which can be dangerous to the user of the mill and which may cause uncertain dilutions and undesirable changes in the seed. A method for dry milling soybean seed was developed in this study to process seed material for assay, including 15N isotope ratio analysis. The method involved the use of a sealed grinding device and dry ice. The seed and ice are ground together for 2 min. The resulting meal is similar in texture and recoverability to that obtainable by grinding in a solvent, and the problem of cross contamination is minimal.

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