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This article in AJ

  1. Vol. 66 No. 5, p. 636-639
     
    Received: Oct 20, 1973
    Published: Sept, 1974


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doi:10.2134/agronj1974.00021962006600050010x

Isolation of β-Nitropropionic Acid from Crownvetch1

  1. D. L. Gustine,
  2. J. S. Shenk,
  3. B. G. Moyer and
  4. R. F. Barnes2

Abstract

Abstract

Compounds that are toxic when fed to meadow voles (Microtus pennsylvanieus) have been isolafed from freshcut, oven-dried crownvetch (Coronilla varia L.) forage. This activity was detected by weanling meadow vole bioassays in which weight change, diet intake, and death were recorded during 6-day feeding trials. The compounds were extracted from dried, ground forage with 95% ethanol. Nonpolar compounds were precipitated by addition of water, and the active compounds remained in aqueous solution. The active compounds remained in the aqueous phase after partitioning with chloroformethanol (8.5:1.5), but were extracted into the organic phase during partitioning with ethyl formate. They were further purified by passage through a polyamide column, and then fractionated by silicic acid column chromatography. From the final fractions, β-nitropropionic acid (BNPA) was isolated. This is the first report of the presence of BNPA in crownvetch. Weanling voles fed crownvetch and BNPA ~in diets at 20 and 0.15%, respectively, showed identical responses, thus indicating that BNPA is a toxic factor in crownvetch.

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