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This article in AJ

  1. Vol. 63 No. 3, p. 515-516
     
    Received: Nov 12, 1970
    Published: May, 1971


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doi:10.2134/agronj1971.00021962006300030053x

Rapid Transectioning of Plant Leaves1

  1. Harold W. Gausman and
  2. Marcia Schupp2

Abstract

Abstract

Photomicrographs of oleander leaf transections (Nerium oleander L.) were taken within 15 min after leaves were excised from the plant and sectioned with a Hooker plant microtome. This rapid procedure, compared with the approximately 20-day conventional method involving tissue fixation, paraffin embedding, staining, and sectioning with a rotary microtome, apparently gave a truer representation of the leaf mesophyll arrangement (particularly size of intercellular spaces), and usually gave better definition to leaf cuticles, druses, multiple epidermal layers, trichomes, and stomates within stomatal crypts. Variability among leaf sections, however, was greater for the Hooker than for the conventional method. Discounting some disadvantages, the rapid procedure should be useful in a portable field laboratory for “on-the-spot” sectioning and photomicrographic recording of plant tissue development.

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